Human Waste Research Articles

Characterization and Performance Evaluation of Anaerobic Night Soil Biodegrading Inoculum

The efficiency of anaerobic night soil (human excreta) biodegradation depends on the characteristics of the inoculum used. This anaerobic microbial inoculum (AMI) contains a microbial consortium, including rate-limiting methanogens whose growth depends on various physicochemical and biological parameters necessitating the inoculum characterisation essential for optimum night soil (NS) biodegradation. The inoculum contained 24.567 g/L (±0.07) total solid, 5.022 (±0.57) g/L total dissolved solid, 18.148 (±0.0.058) g/L volatile solid (VS), and 7.0±0.2 pH. The Gas chromatography analysis confirmed the presence of a small amount of volatile fatty acid (VFA), (≤ 4 mM except propionate) showing rapid conversion of VFA to methane, and 55% methane. Further, the presence of methanosaetaceae group of methanogens was detected through Taqman probe-based real-time PCR. The inoculum performance was evaluated for NS biodegradation in semicontinuous mode of feeding in four 2 L anaerobic biodigesters (2D HRT, 4D HRT, 5D HRT, and 10D HRT) with varying hydraulic retention time (HRT) (2, 5, 8, and 10 days, respectively) at mesophilic temperature (35°C). After 5 HRT, VS reduction for 2D HRT and 5D HRT was <40% while for 8D HRT and 10D HRT was >45-50% and >45-60%, respectively. The methane content was <20% in biogas for 2 and 5 days HRT while for 8 and 10 days >45-50% and 50-65% methane, respectively. The % VS reduction and methane content showed the overfeeding of NS in biodigesters 2D HRT and 5D HRT while 8D HRT showed towards optimum and 10D HRT optimum with improved VS reduction and methane content.

A Comphrensive Review on Amoebiasis

With its wide range of dissemination, Entamoeba histolytica poses a serious threat in practically any nation where there is a regular barrier between human waste, food, and water sources. Except E. histolytica, at least eight distinct amoebas are thought to be commensals in the human intestinal lumen. As the second biggest cause of death from parasitic disease globally, the parasite poses a serious threat to public health in most regions of the world and has reemerged in several previously inactive locations. The majority of E. histolytica infections have very minor or no symptoms, however, colitis and liver abscesses brought on by amoebic infections are the most common clinical manifestations. Typically, a laboratory's diagnosis of amoebiasis is based on a microscope.

Manual Prático do ódio by Ferréz: Waste in the Favela’s Underworld

This article analyzes the work Manual Prático do ódio by Ferréz in the light of some theories that deal with what is relegated to the margins. The aim is to demonstrate that in Ferréz's work all the characters, in their situation of marginalized subjects, show the condition of human waste, being both lagging victims of economic progress and victims of stigmas and diatribes manifested by hierarchically superior beings. The research considers Zygmunt Bauman's concept of human waste as its backbone, an approach that is intertwined with secondary sources (Butler, Lipovetsky, Lynch, Paugam, Silva-Santisteban, Wacquant) referring to other forms and dimensions of marginality.

Prinsip Pariwisata Berbasis Masyarakat dalam Pengembangan Desa Wisata Liang Ndara Kabupaten Manggarai Barat

The purpose of the study is to understand and find out how the application of community-based tourism principles in the development of tourism Liang Ndara Tourism Village, West Manggarai Regency. The type of research used is descriptive qualitative research. Descriptive qualitative research is a type of research that aims to describe or describe completely about a phenomenon or situation being studied with actual facts. This research uses qualitative methods that are descriptive. The results of the study showed that Liang Ndara Tourism Village in West Manggarai Regency offers natural, cultural, and handicraft potential. Initially, its development was faced with challenges of limited human resources, funding, and waste management. Local communities are increasingly involved in tourism development, although it is difficult to change the initial mindset from the agricultural sector to tourism. The village applies 5 principles of community-based tourism, of which 3 have been well implemented. Positive impacts include new jobs, cultural assimilation, suave lifestyles, increased social interaction, community participation, and solidarity. However, the environmental impact still needs further attention, while the political impact is already visible. Efforts are ongoing to reduce negative impacts and increase benefits to local communities.

Assessment of Biomass Energy Potential for Biogas Technology Adoption and Its Determinant Factors in Rural District of Limmu Kossa, Jimma, Ethiopia

Increasing clean energy access for the rural population of developing countries is a priority to meet the United Nation’s Sustainable Development Goals-Zero hunger and affordable modern/clean energy for all. Similarly, to meet this goal, Ethiopia moved towards the development of renewable energy. However, there is a limited knowledge on the biomass energy potential for biogas technology adoption at the local/district level. Thus, this study aimed at assessing the biomass energy potential for biogas technology adoption and its determinant factors among rural households in Limmu Kossa district, Ethiopia. Data was collected from 411 households from 13–24 June 2021. The quantitative data was analyzed using Statistical software Package for Social Science (SPSS) version 23 and Microsoft Word-Excel. The qualitative data was analyzed using content analysis. The study showed that over 96% of households rely on the traditional use of biomass energy for cooking. Nevertheless, on average, about 1 m3 of biogas energy can be potentially available from livestock dung and human excreta per household per day. However, the huge potential of biomass energy did not contribute to improved energy technologies such as biogas. The adoption of biogas is hampered by the non-functionality of the installed biogas, a lack of awareness, the availability of firewood, and the socio-economic characteristics of the households. Thus, improving the awareness of the community, arranging financial access, and training biogas technicians, especially from the local community, would increase the adoption of the technology. However, meeting the digester water demand with the water collected from the walking distances of 15–20 min can be challenging. Community-based biogas digesters or biogas involving income generation with a water supply around the digester would be a better and more sustainable option for biogas energy adoption and use.

Sources of NH4+ in PM2.5 and Their Seasonal Variations in Urban Tianjin China: New Insights From the Seasonal δ15N Values of NH3 Source

AbstractThe stable nitrogen isotopic composition (δ15N) has been widely used to quantify sources of ammonium (NH4+) in PM2.5. However, the overlap and uncertainty in δ15N values from different NH3 sources, coupled with their seasonal variability, hinder accurate identification of NH4+ source. Here, the δ15N values of various NH3 source samples collected by the active sampler were determined. Subsequently, we measured the δ15N values of NH4+ in PM2.5, which were collected seasonally in Tianjin. We found that the combustion‐related NH3 (c‐NH3) exhibiting higher δ15N values compared to volatile NH3 (v‐NH3), but all δ15N values was fell within the range reported by previous studies. Furthermore, inconsistent seasonal variations were observed in the δ15N‐NH3 values originating from emissions of agricultural soil and human excreta. The application of the Bayesian isotope mixing model (MixSIAR model) revealed a significant increase in the contribution of v‐NH3 to NH4+ when incorporating current source data, as opposed to previous data, for δ15N of NH3 source. Notably, the contribution of v‐NH3 (53.1%) to NH4+ was almost equivalent to that of c‐NH3 (46.9%) when considering the seasonal δ15N signatures of NH3 source. Additionally, the estimated contribution of v‐NH3 to NH4+ exhibited significant seasonal variability, which is more reasonable than in the non‐seasonal scenario. This study demonstrated that v‐NH3 and c‐NH3 contributed to NH4+ in PM2.5 in Tianjin almost equally, and it is highlighted that the seasonal δ15N values of NH3 sources should be considered when estimating the contributions of different NH3 sources to NH4+ in PM2.5 by the MixSIAR model.

Analysis of urban metabolism in an informal settlement using the MuSIASEM method in Lima

By 2050, 68% of the global population will reside in cities, driving rapid urban growth and intensifying demand for scarce ecological resources within the Water-Food-Energy nexus. Social metabolism quantifies energy and material transformations with a social focus, building upon urban metabolism. Its application in resource-scarce informal settlements (ISs) has the potential to enhance their sustainability significantly. As community dynamics evolve, acknowledging society as a dynamic variable within this framework becomes increasingly relevant. Our study employs the Multi-Scale Integrated Analysis of Societal and Ecosystem Metabolism (MuSIASEM) framework, focusing on key variables: human activity, land use, money, energy, water, waste, and food. Based on surveys, interviews, GIS datasets, and statistical information, the study investigates the Ciudad de Gosen IS in Lima, Peru. The results show that, in the socio-economic dimension, 43% of the time employed is directed to the unpaid work sector. Notably, 71% of women and 29% of men spend a mean of 44 h/week/person caring for children or elderly. In the paid work sector, there are gender asymmetries; men have a salary 54% higher than women. In the ecological dimension, more than 78% of the homes have access to basic services, unlike other informal settlements in Latin America and Africa.

Temporal evolution of nitrate in Meoqui-Delicias aquifer in Chihuahua, Mexico

The continued input of nitrate (NO3) into groundwater is a global problem, mainly associated to excess fertilizer and improper disposal of human and livestock waste. Nitrate accumulation in oxic aquifers of semiarid areas makes these zones especially susceptible to pollution. Nitrate in Meoqui-Delicias aquifer, located in an important irrigation district in Chihuahua, Mexico, was quantified in 2021 in 63 drinking water wells.  Samples collected were analyzed in laboratory and results were compared to 2003 and 2006 data available for those wells. Nitrate values varied from 0.7 to 23.2 mg/L and 22 % of the wells contained NO3 above drinking water guidelines (10 mg NO3-N/L). A low to moderate nitrate pollution index (NPI) and a slight NO3-N variation with time was observed for most wells. Values of NO3-N/Cl < 1.0 support an anthropogenic origin of nitrate. No association was found between NO3-N and well depth. The most susceptible areas to nitrate contamination were identified as those areas with high NO3-N and increasing concentration with time. The lack of a pattern of contamination suggested leakage of manure leachate at a few points as the most likely contamination source. The consistently high NO3-N content (>10 mg/L) in three deep wells constitutes a serious concern.  DOI: https://doi.org/10.54167/tch.v18i1.1415

Is food self-sufficiency possible for Reunion Island?

In the context of political and economic instability, food self-sufficiency of countries and territories is becoming a burning issue. Reunion Island is a densely populated small French territory isolated in the middle of the Indian Ocean. The previously developed GRAFS model, allowing consistent balances to be established using nitrogen (N) as a common metric for all crops and foodstuffs, has been applied to Reunion Island, considering 11 sub-regions to account for landscape variety. Reunion Island dedicates 87% of its crop production in terms of harvested proteins to the exportation of sugar and tropical fruits, while it imports 67% of its food supply, 54% of livestock feed, and 57% of all fertilizing N inputs to agricultural soils. Overall, the supply of 1 tonne of N as food requires the import of 2.7 tonnes of N as food, feed, and fertilizers. The model also demonstrated that the simultaneous operation of three levers of change would make it possible to reach self-sufficiency in terms of food, feed, and fertilizer: (1) the generalization of agro-ecological crop rotations alternating grain and forage legumes, cereal, and other food crops; (2) the reconnection of livestock with crop farming and a better recycling of manure as well as of human excreta; (3) a drastic reduction of animal-based food in the Reunionese diet, down to 20% of animal products in the total per capita protein intake, instead of the 60% current share. The area dedicated to sugarcane cultivation should be reduced to 15–25% of its current value.

Recycling composted human feces as biofertilizer for crop production: Assessment of soil and lettuce plant tissue contamination by Escherichia coli and human adenovirus

Using waste from sewage systems, particularly human excreta, could save resources and increase soil fertility, contributing to nutrient management. However, because of the pathogenic content in human feces, this resource can pose health risks to farmers and consumers. Therefore, this work analyzed the behavior of the microorganisms: Escherichia coli ATCC13706 and human adenovirus (HAdV-2) in the soil and the internal part of the plant tissue during the vegetative stage after applying spiked composted human feces as biofertilizer. In a greenhouse, we simulated the application of the biofertilizer in lettuce cultivation by spiking three concentrations of E. coli (6.58, 7.31, and 8.01 log10 CFU.g−1) and HAdV-2 (3.81, 3.97, and 5.92 log10 PFU.g−1). As a result, we achieved faster decay in soil at higher concentrations of E. coli. We estimated linear decay rates of −0.07279, −0.09092, and −0.115 days, corresponding to T90s of 13.7, 11.0, and 8.6 days from higher to smaller concentrations of E. coli, respectively. The estimated periods for the inactivation of 4 logarithmic units of E. coli bacteria in soil are longer than the cultivation period of lettuce for all concentrations studied. Concerning the bacterial contamination in plants, we found E. coli in the internal part of the leaves at the highest concentration tested during the first three weeks of the experiment. Furthermore, HAdV-2 was found in roots at a stable concentration of 2–2.3 log10 PFU.g−1 in five of the six samples analyzed. Therefore, bacterial infection could pose a risk, even if fresh greens are washed before consumption, especially for short-term cultures. Regarding viral infection, a positive result in the roots after disinfection may pose a risk to root and tubercule vegetables. These discoveries highlight the importance of conducting comprehensive evaluations of hygiene practices in incorporating organic amendments in crops, explicitly aiming to minimize the risk of post-contamination.

Analyzing recycled waste-infused mortars: Preparation and Examination of thermal, mechanical, and chemical characteristics

In response to the urgent need for innovative and sustainable solutions in the construction industry, this study provides an in-depth investigation and experimental evaluation of the thermal, mechanical, and chemical properties associated with the incorporation of three different categories of waste into mortar. These categories include expanded polystyrene waste (EPSW), expanded perlite waste (EPW), human hair waste (HHW), textile fiber (TFW), and paraffin waste (PW). Various samples were prepared by adding different proportions of these waste materials as sand substitutes in cement mortar. To enable a thorough assessment of the material properties, a comprehensive series of tests were carried out, including measurements of thermal conductivity, flexural strength, compressive strength, chemical composition and crystalline structures. The results revealed that, for a replacement level of 50%, there was a decrease in thermal conductivity by 28%, 55%, 44% and 67% for EPSW, EPW, TFW, HHW and PW mortars, respectively, which resulted in a decrease in compressive strength by 75%, 71%, 50%, 79% and 81%, respectively. Furthermore, the chemical characterization showed that the chemical composition of the mortar remained consistent, even when a substantial proportion of waste materials was introduced. The study emphases the sustainable construction key role in reducing building life cycle emissions.

ADVANCES IN WATER QUALITY ASSESSMENT USING FUZZY APPROACH: RECENT TRENDS OF THEORY AND APPLICATIONS

Due to the rapid growth of economic development and population, the demand for water consumption and the levels of polluted water in Malaysia have constantly increased and are considered crucial issues in Malaysia. Urbanization has steadily produced great amounts of human wastes, such as domestic and industrial wastes which inevitably end up in the water bodies. The World Health Organization claimed that approximately 2.2 million people die annually from such illnesses caused by ingesting bacteria, viruses, protozoans, and other pathogenic microbes in untreated or poorly treated waters or wastewater. This paper intends to provide insight into the publication trends on water quality assessment using fuzzy approaches from 2015 to 2023. This study conducted a bibliometric analysis of 404 scholarly works on water quality assessment as recorded in the Scopus database in September 2023. The data was analysed using a variety of software which are VOSviewer, Harzing’s Publish and Perish, and Microsoft Excel. This paper highlights important contributions, publications, statistics, and current trends of theory and applications in the field. The finding also addresses essential information on present and future developments and critical information for increasing the inclusivity and global reach of water quality assessment which need further attention from the fuzzy research community.

Molecular Identification and Subtype Analysis of Blastocystis sp. Isolates from Wild Mussels (Mytilus edulis) in Northern France.

Blastocystis sp. is the most common single-celled eukaryote colonizing the human gastrointestinal tract worldwide. Because of the proven zoonotic potential of this protozoan, sustained research is therefore focused on identifying various reservoirs of transmission to humans, and in particular animal sources. Numerous groups of animals are considered to be such reservoirs due to their handling or consumption. However, some of them, including mollusks, remain underexplored. Therefore, a molecular epidemiological survey conducted in wild mussels was carried out in Northern France (Hauts-de-France region) to evaluate the frequency and subtypes (STs) distribution of Blastocystis sp. in these bivalve mollusks. For this purpose, 100 mussels (Mytilus edulis) were randomly collected in two sampling sites (Wimereux and Dannes) located in the vicinity of Boulogne-sur-Mer. The gills and gastrointestinal tract of each mussel were screened for the presence of Blastocystis sp. by real-time polymerase chain reaction (qPCR) assay followed by direct sequencing of positive PCR products and subtyping through phylogenetic analysis. In parallel, sequences of potential representative Blastocystis sp. isolates that were previously obtained from temporal surveys of seawater samples at marine stations offshore of Wimereux were integrated in the present analysis. By taking into account the qPCR results from all mussels, the overall prevalence of the parasite was shown to reach 62.0%. In total, more than 55% of the positive samples presented mixed infections. In the remaining mussel samples with a single sequence, various STs including ST3, ST7, ST14, ST23, ST26 and ST44 were reported with varying frequencies. Such distribution of STs coupled with the absence of a predominant ST specific to these bivalves strongly suggested that mussels might not be natural hosts of Blastocystis sp. and might rather be carriers of parasite isolates from both human and animal (bovid and birds) waste. These data from mussels together with the molecular identification of isolates from marine stations were subsequently discussed along with the local geographical context in order to clarify the circulation of this protozoan in this area. The identification of human and animal STs of Blastocystis sp. in mussels emphasized the active circulation of this protozoan in mollusks and suggested a significant environmental contamination of fecal origin. This study has provided new insights into the host/carrier range and transmission of Blastocystis sp. and emphasized its potential as an effective sentinel species for water quality and environmental contamination.

COVID-19 trends at the University of Tennessee: predictive insights from raw sewage SARS-CoV-2 detection and evaluation and PMMoV as an indicator for human waste.

Wastewater-based epidemiology (WBE) has become a valuable tool for monitoring the prevalence of SARS-CoV-2 on university campuses. However, concerns about effectiveness of raw sewage as a COVID-19 early warning system still exist, and it's not clear how useful normalization by simultaneous comparison of Pepper Mild Mottle Virus (PMMoV) is in addressing variations resulting from fecal discharge dilution. This study aims to contribute insights into these aspects by conducting an academic-year field trial at the student residences on the University of Tennessee, Knoxville campus, raw sewage. This was done to investigate the correlations between SARS-CoV-2 RNA load, both with and without PMMoV normalization, and various parameters, including active COVID-19 cases, self-isolations, and their combination among all student residents. Significant positive correlations between SARS-CoV-2 RNA load a week prior, during the monitoring week, and the subsequent week with active cases. Despite these correlations, normalization by PMMoV does not enhance these associations. These findings suggest the potential utility of SARS-CoV-2 RNA load as an early warning indicator and provide valuable insights into the application and limitations of WBE for COVID-19 surveillance specifically within the context of raw sewage on university campuses.

Urine-enriched biochar: Coupling sustainability in sanitation and agriculture

Linear models of fertilizer production and application are environmentally harmful. Predominant approaches to waste management treat human excreta as a pollutant rather than a source of nutrients for agriculture. Container-based sanitation (CBS) systems safely contain and transport excreta for treatment and reuse, though urine is often contained but not treated. A major challenge of urine-nutrient recovery is the shift in nitrogenous species in urine during storage, from urea to ammonia (NH3) and ammonium (NH4+), due to urease activity. This can lead to gaseous NH3 losses from urine that depletes its fertilizer potential. Urine-enriched biochar (UEBC) may act as a slow-release fertilizer of urine nutrients. We quantified the adsorption of nitrogen in fresh, stored, and CBS-style urine to wood waste, sewage sludge, and walnut shell biochars. These UEBCs were compared to urine-only treatments and fertilized and unfertilized controls in a greenhouse growth experiment. We found that the <500-µm biochar size fraction retained significantly more nitrogen than larger particles across biochars. Urine-nitrogen adsorption to biochar and uptake into plant tissue varied across biochar type and urine condition. The quantity of urine applied in urine-only treatments, regardless of type, was positively correlated with plant nitrogen uptake. Plant biomass did not differ significantly across treatments. These findings emphasize the need to optimize UEBC application for different urine and biochar conditions, particularly for CBS and other urine-diverting operations.

Nurturing Eco-Literate Minds: Unveiling the Pathways to Minimize Ecological Footprint in Early Childhood Education

This research explores the vital role of the ecological footprint as a pivotal indicator in ecological assessments and its significance in advancing environmental sustainability. Investigating the intricate dynamics of human activities, resource consumption, waste management, and ecological impact, this study underscores the need for a comprehensive understanding. Key aspects such as renewable energy, economic growth, biomass capacity, and trade policy within the expanding field of energy consumption are thoroughly examined, with data collected from 624 randomly selected pupils. The research methodology involved pre- and post-activity knowledge assessments. The research places a particular focus on the innovative examination of early childhood education as a potent tool for reducing the ecological footprint. Drawing on insights from prior research, the authors argue that instilling ecological awareness and sustainable practices at a young age can yield lasting benefits. This article highlights successful collaboration between higher education institutions and elementary schools, specifically through the “Green Ambassadors in the Community” course at the Holon Institute of Technology (HIT). This study not only emphasizes the crucial role of educational institutions in fostering sustainable practices but also advocates for the urgent integration of environmental education into early childhood curricula.

Numerical investigation and dynamics of pollutant dispersion in underground restroom ventilation

Urbanization is expanding in both surface and underground space, where the air quality in underground space restrooms in public transportation hubs is a huge challenge due to variations in passenger’ flow and specific features. The pollution in the underground restrooms mainly comes from the microorganisms and odors of human waste. This paper proposes a back-side air exhaust method for the restroom compartment to enhance pollutants removal efficiency, and an optimal design study is performed to determine the best device position, evacuation pressure, and air exchange rate. In response to research gaps, we introduced the influence of unsteady human breathing behavior and human obstacles coupled with the flow field and the pollutants diffusion in the CFD model. After model verification, we numerically investigated the effect of different ventilation modes. The results show that the top evacuation mode may accelerate the diffusion of pollutants. In contrast, the pollutant concentration around the mouth and the spillage outward from the door cracks are significantly reduced under the new ventilation mode. The standardized and actual concentrations of the pollutant and the normalized exposure index were used to assess the indoor air quality. Finally, we put forward a series of dynamical models to describe the variation of indoor pollutant concentrations over time. The study's results will provide reliable theoretical and numerical support to improve the air quality of public restrooms in urban underground spaces.

Assessments of heavy metal contaminations of surface water and sediment of Pülümür Stream (Türkiye)

The primary considerations within aquatic ecosystems are water and sediment quality, rendering it imperative to assess their concentrations, pollution levels, origins, and ecological hazards. In this study, heavy metal concentrations in surface waters and sediments of the Pülümür Stream were investigated to assess the level, distribution, source of pollution and the associated human risks. Water and sediment samples from the river were collected monthly from February 2021 to January 2022 and examined using the Inductively Coupled Plasma Mass Spectrometry (ICP– MS) technique. Metal concentrations in the sediment followed a decreasing or derivative sequence of Fe > Al > Mn > Ni > Cr > Zn > Cu > Pb > As >Cd > Hg. The ecological indices of the stream water have low degrees of contamination by heavy metals. Igeo and EF showed that most of the sites are moderately to highly polluted due to Ni, and Cr, while PLI and PERI showed a progressive deterioration of the environment at site 9. Moreover, the modified hazard quotient (mHQ) indicates low ecological risk for Cu, Pb, Zn, Cd, Hg metals, while Ni, As and Cr reach high pollution at sediment sites. Pearson correlation analysis revealed that the presence of Zn, Cu, Pb, Hg, and Mn metals in the sediment analysis has a lithogenic source, and As, Ni, Cd, and Cr accumulate due to human activities, mainly domestic and agricultural irrigation wastes and mining wastes, all of which affect the aquatic environment. According to this study, more attention should be paid to the comprehensive monitoring of metals in the water and sediment for both aquatic biota and human risk of the Pülümür River.

An environmental evaluation of urine-diverting dry toilets in Hiloweyn Camp, Dollo Ado, Ethiopia

Safe and hygienic management of human waste is essential in humanitarian settings. Urine-diverting dry toilets (UDDTs) can enable this management in some humanitarian emergency settings. A seeded, longitudinal environmental study was conducted in Hiloweyn refugee camp, Dollo Ado, Ethiopia, to measure Escherichia coli and Ascaris suum ova inactivation within closed UDDT vaults and to document environmental conditions (temperature, moisture content, and pH) that could influence inactivation. Hiloweyn camp represented an optimal location for a desiccation-based sanitation technology such as the UDDT. E. coli and Ascaris ova inactivation was observed in UDDTs under warm, dry, alkaline conditions at 6, 9, and 12 months of storage; UDDTs with samples containing <1000 E. coli/g total solids increased from 30 % to 95 % over 12 months, and a >2.8-log10 reduction in Ascaris ova viability was observed after 6 months. Additional laboratory-based studies were conducted to provide insights into the field study findings and study the impact of hydrated lime on E. coli and Ascaris ova inactivation. Results suggest that adding hydrated lime to elevate pH > 12 may increase inactivation and decrease storage time. Overall, UDDTs could contribute to the safe and hygienic management of human waste in comparable warm and dry humanitarian settings.

A Novel Tiled Amplicon Sequencing Assay Targeting the Tomato Brown Rugose Fruit Virus (ToBRFV) Genome Reveals Widespread Distribution in Municipal Wastewater Treatment Systems in the Province of Ontario, Canada.

Tomato Brown Rugose Fruit Virus (ToBRFV) is a plant pathogen that infects important Solanaceae crop species and can dramatically reduce tomato crop yields. The ToBRFV has rapidly spread around the globe due to its ability to escape detection by antiviral host genes which confer resistance to other tobamoviruses in tomato plants. The development of robust and reproducible methods for detecting viruses in the environment aids in the tracking and reduction of pathogen transmission. We detected ToBRFV in municipal wastewater influent (WWI) samples, likely due to its presence in human waste, demonstrating a widespread distribution of ToBRFV in WWI throughout Ontario, Canada. To aid in global ToBRFV surveillance efforts, we developed a tiled amplicon approach to sequence and track the evolution of ToBRFV genomes in municipal WWI. Our assay recovers 95.7% of the 6393 bp ToBRFV RefSeq genome, omitting the terminal 5' and 3' ends. We demonstrate that our sequencing assay is a robust, sensitive, and highly specific method for recovering ToBRFV genomes. Our ToBRFV assay was developed using existing ARTIC Network resources, including primer design, sequencing library prep, and read analysis. Additionally, we adapted our lineage abundance estimation tool, Alcov, to estimate the abundance of ToBRFV clades in samples.